4Installation
Software Installation
2D-LC User Guide 98
4 Verify the Valves. Depending on the 2D-LC Valve you have installed, the
Standard 2D-LC (G4236A) or the ASM 2D-LC Valve (G4243A) will
automatically appear.
[OPTIONAL]
a If your system contains Multiple Heart-Cutting decks, specify which valve
head is corresponds to Deck A or B respectively will automatically appear.
[OPTIONAL]
b If the system contains a diverter valve, specify the role of the valve here.
You can define further diverter valve settings in the method, see “Specify
the Switch Time of the Diverter Valve” on page 143.
5 Verify the Capillaries. Select by clicking your installed capillaries. Check for
correct loop size and correct length of the transfer capillaries. If an ASM
2D-LC Valve is used, define the ASM capillary that defines your split ratio, see
“Introduction to Active Solvent Modulation (ASM)” on page 35.
• To define the Sample Loop e.g. default 40 µL Sample Loop p/n 5067-5926
for MHC or 5067-5425 for SHC
• To define the transfer capillary, e.g., default Capillary 0.12x170 (1.9 µL)
5500-1270 for standard valve or Capillary 0.12x170 (1.9 µL 5500-1376 for
ASM valve
• To define the ASM capillary, e.g., default Capillary 0.12x170 (1.9 µL
5500-1301 for ASM valve, ASM factor 3
6
To finish, leave the 2D-LC Cluster Configuration, get to the next window, click
OK
.
Not all detectors to be configured will automatically appear in the configuration
window. If you want to configure more detectors, you have to do it manually
using the add-on button. Please note the following notation first the module
number followed by a colon and then the serial number, for example G6135C:
US12345678.
The detector entry like module number and serial number must be correct to
avoid issues later.
For the MassHunter workflow with a High-End mass spectrometer as another
second detector, the detector is usually not visible here. Define this transfer
volume (delay) during the file splitting in the data evaluation, see “Automated File
Splitting” on page 231.
The selection of the ASM Capillary determines the ASM factor, see “Introduction
to Active Solvent Modulation (ASM)” on page 35.
Therefore the ASM factor value is read only.
Generic capillaries are allowed but must be configured first in Lab Advisor before
they show up here, see “2D-LC Capillaries Configuration Tool” on page 283.
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