Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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Caution If the sample temperature is critical for accuracy, experiments should be
performed to validate water bath settings. Actual heat transfer varies with vial
material, humidity, condensation on the vial, and flow rates.
To calculate the temperature needed for the water bath, complete the next four
tasks. To convert temperature units, use the formula °C = 5/9 (°F – 32) or
°F = 9/5(°C) + 32.
1. Measure the average ambient air temperature near the tray.
2. Find the desired sample vial temperature on the y-axis of the graph below.
3. From the point you selected in step 2, draw a horizontal line over to the
diagonal line that corresponds with the ambient air temperature you
measured in step 1.
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