Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
172
4. From the intersection of desired vial temperature and ambient
temperature, draw a vertical line down to the x-axis of the graph and read
the temperature needed for the water bath.
Figure 88. Sample vial temperature versus water bath temperature for values of
ambient air temperature
Tray quadrant temperatures and pressures
Figure 88 shows the maximum pressures and temperatures measured at the
output to the quadrant plumbing.
For example, with a water bath temperature of 40°C, the output pressure to the
tray quadrants should not exceed 3.5 psi.
50
40
30
20
10
0
5030
20
100
Ambient
Temperature
40 C
30 C
25 C
20 C
15 C
10 C
Desired Vial
Temperature
(˚C)
(y-axis)
40
Temperature of Water Bath (˚C)
(x-axis)
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