Moor Instruments moorLDI2-VR - Page 123

MOOR INSTRUMENTS moorLDI2 RESEARCH USER MANUAL
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The table below shows scaling constants for the default distance factor, 1.2 (DF|dc):
New MK1 & MK2 Scanner Distance
Tables - Multipliers
Distance DF|none DF|dc DF|dc2
10 4.56
1.35
0.37
15 3.12
1.20
0.50
20 1.90
1.10
0.68
25 1.19
1.05
0.87
30 1.00
1.00
1.00
35 1.00
1.01
1.00
40 1.04
1.03
0.97
45 1.08
1.04
0.86
50 1.25
1.06
0.79
55 1.46
1.07
0.70
60 1.76
1.09
0.63
65 2.03
1.10
0.55
70 2.35
1.11
0.49
75 2.70
1.13
0.43
80 3.10
1.14
0.37
85 3.48
1.16
0.34
90 3.90
1.17
0.31
95 4.39
1.19
0.27
100 4.88
1.20
0.25
105 5.40
1.21
0.22
110 5.95
1.23
0.20
115 6.50
1.24
0.19
120 7.30
1.26
0.16
Note for dc normalisation the flux decreases as the measurement distance increases from 30cm to
120cm but for dc squared normalisation it increases - an increase of approximately 4 at 100cm (this
requires a distance factor multiplier of X0.25).
Note : all moorLDI's have the same distance factor multiplier look up tables for dc squared and
unnormalised flux. These are only an approximation. If you are using either of these conditions and
you wish to compare measurements taken at different distances you must take measurements at the
two distances using the motility standard. Comparisons can then be made and the appropriate
multiplier used in post detection processing (see Figure below).
For dc normalisation the default distance factor table is a good approximation; however to achieve
more accurate results each instrument can have the 100cm multiplier entered via the service software
(done at manufacture post March 2002). For instruments manufactured before March 2002 this could
not be done; however software is now (March 2002) available.