Moor Instruments moorLDI2-VR

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MOOR INSTRUMENTS moorLDI2 RESEARCH USER MANUAL

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Default Chapter3
- Table of Contents3
1 Safety8
- Introduction and Intended Use8
- Laser Safety8
  - LASER SAFETY Moorldi2-VR and Moorldi2-HR9
  - LASER SAFETY Moorldi2-IR and Moorldi2-HIR11
- Beam Delivery System14
- Laser Warning Labels15
- Electrical Safety Labels16
- Environmental Conditions17
- Warnings / Caution Do's and Don'ts17
2 System Description18
3 Moorldi2 Unpacking and Assembly22
- Stand Assembly22
- Unpacking the Scan Head24
- Installation of F6C Framegrabber Card (Analogue Camera Model)24
- Installation of Firewire Card (Firewire Camera Model)25
- Cable Connections26
  - Cabling26
  - Mobile Stand and Cabling33
- The Computer and Software Installation34
  - Computer Specifications34
  - Software Installation and Registration34
- Before First Use39
- Transportation39
4 The Laser Doppler Technique40
- Introduction40
- Spatial Variation of Perfusion40
- Principles of the Laser Doppler Imaging Technique40
- ELEMENTS of the Moorldi241
- The Laser Doppler Processing Algorithm42
5 Introduction to Moorldi2 Software44
- Overview45
- GETTING STARTED with the Moorldi246
- Changing Scan Parameters47
- Moorldi2-Hr and Moorldi2-Hir Scan Distance and Scan Speed47
6 Moorldi2 Set up48
- Introduction48
- Moorldi2 IMAGE SCAN CONFIGURATION49
- Scanner Set-Up49
- Setup: Preference Setup59
7 Single Image Scan61
- To Start61
- Saving an Image Scan62
- Image Display in Measurement Mode62
8 Repeat and Line Scan Measurement64
- Introduction64
- Repeat and Line Scan Set up64
  - Repeat Interval64
  - Number of Scans64
- Repeat Scan Start65
- Repeat Scan Display During Measurement66
- Repeat Line Scan Start67
- Iontophoresis67
9 Single Point Measurement (Spm)68
- Introduction68
- Positioning of Beam for Spm68
- Beam Movement68
- Beam Movement Relative to Photo Image68
- Single Point Measurement Window68
- DISTANCE - Moorldi2-TO-TISSUE69
- Single Point Configuration69
- Autogain70
- Single Point Measurement - Start70
- Single Point Measurement - File Store71
10 Image Review72
- Introduction72
- Summary of Image Processing Utilities72
- Opening Files and Image Display75
- View76
- PREFERENCE OPTIONS (for Image Review)80
11 Image Processing81
- Reload81
- Interpolate81
- Normalise81
- Rotate or Flip82
- Adjust Settings82
  - Distance82
  - Background Threshold82
- Smooth83
- Average83
- Subtract83
- Threshold/ Cut85
12 File86
- Saved Image File Formats86
- Redefine Ldi File87
- Print Report88
13 Repeat Scan Processing89
- Introduction89
- Repeat Scan Display89
- Separate Image Display90
- Re-Define Repeat File90
14 Region of Interest91
- Introduction91
- Region of Interest (Roi): Roi Toolbar91
- Defining Roi' - General92
- Zoom in92
- Polygon Roi92
- Circular Roi93
- Saving Roi93
15 Image Analysis94
- Introduction94
  - Types of Image Analysis94
  - Pixel Values94
- Statistics - Single Image Scan95
- Histogram96
- Profile99
16 Repeat Image Analysis102
- Introduction102
- Saving Results of Repeat Images106
- Repeat Line Analysis106
17 Trace Review (Single Point Measurements)108
- Introduction108
- Single Point Measurement Display108
- Trace Scrolling110
- Spm Analysis110
- Spm Trace Printout111
- Spm Statistics Printout111
- Spm Statistics - Saving Results111
18 Troubleshooting112
- Flux Image During Acquisition112
- Slow Computer Response112
- Loss of Communication with Computer113
19 Calibration and Stability115
- Moorldi V1.0 CALIBRATION GUIDE115
  - Set-Up115
  - Calibration115
- Moorldi V2.0 CALIBRATION GUIDE117
- The Distance Factor121
- Stability Testing125
20 Maintenance126
- Laser Output Power126
- Laser Beam Properties126
- Mechanical Checks126
- Operation of the Solenoid Actuated Optical Attenuator127
- Leads127
- Cleaning127
- Calibration and Stability128
21 Electromagnetic Compatability129
- Cabling129
- Guidance and Manufacturer's Declaration - Electromagnetic Emissions129
- Guidance and Manufacturers Declaration - Electromagnetic Immunity130
22 Specifications133
23 Returns Procedure137
- Decontamination137
24 Warranty and Service Policy138
- Service Due Warning138
25 Accessories139
26 Disposal140
Appendix 1. Preparing Your Moorldi2 Imager for the New Software141
Appendix 2. Moorldi/Iontophoresis User Manual V5150
- Introduction150
- INSTRUCTIONS for USE of Moorldi / IONTOPHORESIS150
- Pause Function152
- Manual Iontophoresis152
- Protocol Analysis153
- Recommendations and Protocols154
Appendix 3. Printouts from Processing156
Index161

Moor Instruments moorLDI2-VR - Page 37

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