CHAPTER 11 Technical Information
11-16 Sysmex XE-5000 Instructions for Use
Revised April 2007
HGB Analysis procedure
During an HGB analysis, the amount of hemoglobin in the
blood is analyzed. The procedure for analyzing HGB is
explained as follows:
1. Blood is aspirated from the manual aspiration pipette to
the sample rotor valve.
2. 3.0 µL of blood, measured by the sample rotor valve, is
diluted to a ratio of 1:333 with 0.9970 mL of CELLPACK,
and then sent to the flow cell as the diluted sample.
At the same time, 0.5 mL of SULFOLYSER is added to
hemolyze the red blood cells to make 1:500 diluted
sample, and the hemoglobin is converted into SLS-
hemoglobin.
3. Light (of wavelength 555 nm) emitted from the light-
emitting diode passes through the lens and into the
sample in the Hgb cell. The concentration of
SLS-hemoglobin is analyzed as light absorbance, and is
calculated after substruction of the absorbance of the
diluent measured before the sample was added.
Sample rotor valve
Photodiode
HGB analysis reagent 1
CELLPACK: 1.9960 mL
HGB analysis reagent 2
SULFOLYSER: 0.5 mL
Sample tube
(blood: 3.0 µL)
HGB analyzer
Flow cell
Light-emitting
diode (LED)
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