CHAPTER 11 Technical Information
Sysmex XE-5000 Instructions for Use 11-15
Revised April 2007
3. RBC/PLT and HGB analysis
RBC/PLT analysis procedure
During RBC and PLT analysis, the red blood cells and
platelets in the blood are analyzed. The procedure for
analyzing RBC/PLT is explained as follows:
1. Blood is aspirated from the manual aspiration pipette to
the sample rotor valve.
2. 4.0 µL of blood, measured by the sample rotor valve, is
diluted to a ratio of 1:500 with 1.9960 mL of CELLPACK,
and then sent to the RBC sample chamber as the diluted
sample.
3. The sheath injector piston sends 11.7 µL of diluted
sample slowly to the RBC/PLT detector.
4. The RBC detector counts the RBC and PLT via the Hydro
Dynamic Focusing (DC Detection).
At the same time, the hematocrit (HCT) is calculated via
the RBC pulse height detection method.
RBC sample chamber
RBC/PLT analysis reagent 2
CELLSHEATH: 2.1 mL
RBC/PLT analysis reagent 1
CELLPACK: 1.9960 mL
Sample rotor valve
Sample tube
(blood: 4.0 µL)
RBC/PLT detector
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