SYSMEX XE-5000 - Page 236

CHAPTER 11 Technical Information
11-18 Sysmex XE-5000 Instructions for Use
Revised April 2007
4. WBC classification
White blood cells (leukocytes) can be broadly classified as
either lymphocytes, monocytes or granulocytes. Granulocytes
can be further classified as either neutrophils, basophils or
eosinophils, depending on the dye-affinity of the granules.
The applicable analysis procedure is explained as follows:
4DIFF analysis procedure
A 4DIFF analysis is used to identify and analyze the following
white cell subpopulations: lymphocytes, monocytes, eosinophils,
neutrophils, basophils and immature granulocytes. The 4DIFF
analysis procedure is explained as follows:
1. Blood is aspirated from the manual aspiration pipette to
the sample rotor valve.
2. 18 µL of blood, measured by the sample rotor valve, is
diluted with 0.882 mL of STROMATOLYSER-4DL, and
then sent to the reaction chamber as the diluted sample.
At the same time, 18 µL of STROMATOLYSER-4DS is
added to dilute the sample to a ratio of 1:51. After reacting
for about 22 seconds in this condition, the red blood cells
are hemolyzed and the white blood cells are stained.
3. The sheath injector piston sends 40 µL of diluted sample
to the optical detector block.
4. In the optical detector block, the sample is analyzed via
flow cytometry method utilizing a semiconductor laser.
STROMATOLYSER-4DL:
0.882 mL
STROMATOLYSER-4DS:
18 µL
Sample rotor valve
Sample tube
(blood: 18 µL)
Reaction chamber
Optical detector
block
CELLPACK