SYSMEX XE-5000 - Page 237

CHAPTER 11 Technical Information
Sysmex XE-5000 Instructions for Use 11-19
Revised April 2007
WBC/BASO analysis procedure
A WBC/BASO analysis is used to analyze the number of
white blood cells, as well as the number of basophils at the
same time. The WBC/BASO analysis procedure is explained
as follows:
1. Blood is aspirated from the manual aspiration pipette to
the sample rotor valve.
2. 18 µL of blood, measured by the sample rotor valve, is
diluted to a ratio of 1:50 with 0.882 mL of
STROMATOLYSER-FB, and then sent to the reaction
chamber as the diluted sample.
After reacting for about 14 seconds in this condition, the
red blood cells are hemolyzed.
3. The sheath injector piston sends 40 µL of diluted sample
to the optical detector block.
4. In the optical detector block, the sample is analyzed via
flow cytometry method utilizing a semiconductor laser.
Sample tube
(blood: 18 µL)
STROMATOLYSER-FB:
0.882 mL
CELLPACK
Sample rotor valve
Reaction chamber
Optical detector
block