CHAPTER 11 Technical Information
Sysmex XE-5000 Instructions for Use 11-21
Revised April 2007
5. WBC differential analysis
4DIFF and WBC/BASO scattergrams
Forward scattered light, lateral scattered light and lateral
fluorescent light are detected via fluorescence flow cytometry
method utilizing a semiconductor laser, and two-dimensional
scattergrams are displayed.
In the 4DIFF scattergram, the X-axis represents the intensity
of the lateral scattered light and the Y-axis the intensity of the
lateral fluorescent light.
After scattergram Analysis, the 4DIFF scattergram displays
the following classified populations: red blood cell ghosts,
lymphocytes, monocytes, eosinophils, immature
granulocytes(IG), neutrophils and basophils.
In the WBC/BASO scattergram, the X-axis represents the
intensity of the lateral scattered light, and the Y-axis the
intensity of the forward scattered light.
The WBC/BASO analysis scattergram displays the classified
populations of red blood cell ghosts, basophils and other
remaing white blood cells (lymphocytes + monocytes +
neutrophils + eosinophils).
WBC/BASO4DIFF
Monocytes
Lymphocytes
Neutrophils
+
Basophils
Lymphocytes + Monocytes +
Neutrophils + Eosinophils
RBC
ghosts
Eosinophils
Basophils
RBC ghosts
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