SYSMEX XE-5000 - Page 243

CHAPTER 11 Technical Information
Sysmex XE-5000 Instructions for Use 11-25
Revised April 2007
9. RET scattergram
After fluorescence flow cytometry analysis, a two-dimensional
distribution of the forward scattered light and lateral
fluorescent light is displayed as a scattergram.
In the scattergram, the X-axis represents the intensity of the
lateral fluorescent light and the Y-axis the intensity of the
forward scattered light.
Scattergram analysis algorythms separate the mature red
blood cells, reticulocytes and platelets from each other and
calculate reticulocyte ratio’s, reticulocyte count, optical red
blood cell count and platelet count.
Here, the scattergram is divided into three RET zones based
on the intensity of the fluorescent light and the ratio of the
reticulocytes in each zone to the total number of reticulocytes
is calculated.
Analysis parameters
Reticulocyte Ratio:
Reticulocyte Count:
Low Fluorescence Ratio:
Middle Fluorescence Ratio:
RET
Mature red blood cells
Platelets
Reticulocytes
RET
LFR MFR HFR
P
art
i
c
l
e count
i
n ret
i
cu
l
ocyte zone
RET% = ✕ 100
Particle count in mature RBC zone +
Particle count in reticulocyte zone
RET% ✕ RBC
RET# =
100
LFR = 100 – HFR – MFR
P
art
i
c
l
e count
i
n
MFR
zone
MFR = ✕ 100
Particle count in reticulocyte zone