SYSMEX XE-5000 - Page 242

CHAPTER 11 Technical Information
11-24 Sysmex XE-5000 Instructions for Use
Revised April 2007
8. RET analysis
RET analysis procedure
A RET analysis is used to classify and analyze groups of red
blood cells, platelets, reticulocytes and reticulocyte
sub-population in the blood. The RET analysis procedure is
explained as follows:
1. Blood is aspirated from the manual aspiration pipette
through the sample rotor valve.
2. 4.5 µL of blood, measured by the sample rotor valve, is
diluted with 0.8955 mL of RET SEARCH (II) diluent, and
then sent to the reaction chamber as the diluted sample.
At the same time, 18 µL of RET SEARCH (II) dye solution
is added to dilute the sample to a ratio of 1:204.
After reacting for about 31 seconds in this condition, the
diluted sample is stained and analyzed by fluorescence
flow cytometry.
3. The sheath injector piston sends 2.8 µL of diluted sample
to the optical detector block.
4. In the optical detector block, the sample is analyzed via
fluorescence flow cytometry utilizing a semiconductor
laser.
Sample rotor valve
Optical detector
block
Sample tube
(blood: 4.5 µL)
Reaction chamber
CELLPACK
RET SEARCH (II)
diluent: 0.8955 mL
RET SEARCH (II)
dye solution: 18 µL