Invitrogen EVOS M5000 - Stage View Issues; Software Interface Issues

Stage view issues
Observation
Recommended actions
Cannot locate pinhole for
aligning the stage
Use a low-power objective (10x or less).
Cannot find original pin
locations on the vessel
Note the orientation of your slide or vessel in the vessel holder when
creating pins. Use the same orientation when re-locating pins.
Use a low-power objective (10x or less).
For microtiter plates, confirm plate is oriented with well A1 in the upper
left corner of the vessel holder.
Use Stage View-compliant vessel holders. These vessel holders have
screws in the corners and a spring clip to precisely align the vessel holder
on the stage and make re-locating pins easier. Additionally, the vessel
holders have index marks that align with the stagetop marks to confirm
the correct vessel holder orientation in case of removal and re-installation.
Pin map has substantially
shifted
Check the vessel position in the vessel holder to ensure it is placed tightly
in the reference corner.
Vessel was placed incorrectly before imaging and marking pins. Confirm
vessel and vessel holder were installed correctly before initial imaging and
pin placement.
Confirm the vessel holder thumbscrews are secured finger-tight to ensure
accurate alignment.
Software interface issues
Note: We recommend keeping the EVOS
™
M5000 Imaging System up to date with the latest software.
Observations Recommended actions
Image does not respond to
changes in focus or stage
position
Click Light to return to live observation.
Some of the software controls
are not available
The controls available on the EVOS
™
M5000 Imaging System are
contextual; only the controls relevant for the chosen task will be
available.
Save button does not respond
when clicked
Click Capture first; it is only possible to save an image that is captured.
AppendixATroubleshooting
Stage view issues
A
EVOS
™
M5000 Imaging System User Guide
115