Invitrogen EVOS M5000 - Calibrate the Objectives; Calibrate Objective Magnification

Calibrate the objectives
Calibrate objective magnification allows the calibration of the field of view, parfocality, and
parcentration parameters of the selected objective. When calibrated, parfocality ensures that the
sample stays in focus when the objective is changed.
Note: The pre-installed objectives supplied with the EVOS
™
M5000 Imaging System have been pre-
calibrated. You do not need to calibrate them again unless they are reinstalled after removal from the
instrument.
Calibrate objective magnification
Calibration procedure requires the use of the EVOS
™
M5000 calibration slide (Cat. No. AMEP4720)
supplied with the EVOS
™
M5000 Imaging System. Total time required for objective calibration is
approximately 5 minutes.
Note: For best parfocality and parcentration, calibrate the installed objectives one after the other
without removing the calibration slide.
1.
Mount the EVOS
™
Calibration Slide face down in the slide vessel holder (you can use either of the
2 slots).
2.
Select the objective to calibrate using the Objective selection wheel (“Rear view” on page14).
3.
Go to
Settings4Objectives, click
(Action)
for the newly installed objective, then select
Calibrate to launch the Objective Calibration tool.
4.
Find a calibration circle that fits entirely in the field of view.
Chapter12Instrument care and maintenance
Calibrate the objectives
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EVOS
™
M5000 Imaging System User Guide