Invitrogen EVOS M5000 - Cell Culture - Confluence Controls

8.
Batch Analysis: Allows you to save and apply the analysis parameters to other images that
you have collected and saved in an image folder (see “Batch analysis” on page94 for more
information).
9.
Exit: Exits the Manual Count tool and displays the Review tab.
10.
Save: Saves the analysis results as an image in the selected
file format (see “Save analysis results”
on page92 for more information).
Cell culture – confluence controls
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1.
Analysis tools
: Allows you to toggle between Auto Count, Manual Count, or Cell Culture
(Confluence and Transfection Eciency) tools for image analysis.
2.
Confluence
: Expands or hides the controls for the Confluence tool.
3.
Select Target and Background: Allows you to select representative cell and background areas for
the confluence
measurement.
4.
Sensitivity: Adjusts the algorithm sensitivity to pixel intensity (higher intensity = more pixels
included). Decr
easing the sensitivity reduces the confluence value.
5.
Show Mask: Indicates the areas included in the confluence
measurement.
6.
Mask Color: Selects the mask color.
AppendixBGraphical user interface (GUI)
Cell culture – confluence controls
B
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EVOS
™
M5000 Imaging System User Guide