Invitrogen EVOS M5000 - Auto Count Controls

Auto count controls
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1.
Analysis tools
: Allows you to toggle between Auto Count, Manual Count, or Cell Culture
(Confluence and Transfection Eciency) tools for image analysis.
2.
Select Target and Background
: Allows you to select representative target objects and
background areas for Auto Count.
3.
Split Cells
: Allows you to split multiple objects that have been counted as one into individual
objects based on shape or pixel intensity to increase the count accuracy.
4.
Refine: Selects intensity, area, or circularity by which to refine Auto Count results using the Count
Histogram.
5.
Count Histogram
: Allows you to set pixel intensity, area, or circularity thresholds to refine the Auto
Count results.
6.
Object Count
: Displays the object count based on the Auto Count parameters.
7.
Object color: Selects the color by which the counted objects are identified.
8.
Reset
: Resets the count to 0 and clears the selected targets and background areas.
9.
Batch Analysis
: Allows you to save and apply the analysis parameters to other images that
you have collected and saved in an image folder (see “Batch analysis” on page94 for more
information).
AppendixBGraphical user interface (GUI)
Auto count controls
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EVOS
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M5000 Imaging System User Guide