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Invitrogen EVOS M5000 - APPENDIX A Troubleshooting; Image Quality Issues

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Troubleshooting
Note: For additional technical support, contact your local EVOS
distributor. If you do not have your
distributor information, visit thermofisher.com/evos or contact Technical Support.
Image quality issues
Observation
Recommended action
Transmitted light image is too
dim (at higher magnifications)
Remove the condenser slider, if one is in place.
Specks, dots, or blurs on image Follow the instructions under “Objective lens care” (“Objective lens care”
on page108) to clean the objectives.
Uneven focus across screen
Position the sample so that it lies flat on the stage; ensure that the
sample’s thickness is even.
Ensure that the vessel holder is mounted flat with respect to the
stage.
Diculty focusing on
coverslipped sample on
standard slide
Place the slide so the coverslip is facing up (long working-distance
objectives require a thick optical substrate, and image best through 1.0–
1.5mm of glass or plastic).
Image display is black
Click a light button ( ) onscreen.
Center the sample over the objective.
Verify that the power supply is connected and the power switch is
on.
Image display is red, or red
patches cover parts of the
screen
Dim the illumination until the red highlights disappear to get the
maximum level of brightness without any overexposed areas.
Disable the Highlight Saturated Pixels option in the Settings tab.
A
114
EVOS
M5000 Imaging System User Guide

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