Invitrogen EVOS M5000 - Operation Principles and Technical Overview; LED Illumination; LED Light Cubes

Captured images: 16‑bit monochrome (12‑bit dynamic range) TIFF or PNG; 8‑bit per RGB channel
TIFF, PNG, or JPG.
Output ports: 4‑pin power input port (12VDC, 5A), 2 USB 3.0, 2 USB 2.0
Networking capability: Connection through Windows
™
/SMB network via an Ethernet cable connection
or wirelessly using the supplied Wi-Fi adaptor.
Power supply: AC adaptor with country-specific power cords.
Operation principles and technical overview
LED illumination
The EVOS
™
M5000 Imaging System uses an adjustable-intensity LED light source provided by the
proprietary, user-interchangeable LED light cube (see below). The LED light source is as close as
possible to the objective and the number of optical elements is minimized. High-intensity illumination
over a short light path increases the eciency of fluorophore excitation, providing better detection of
weak fluorescent signals.
In contrast to traditional fluorescence microscopy light sources that use mercury, a toxic carcinogen
requiring special handling and disposal, the LED light source of the EVOS
™
M5000 Imaging System is
more environmentally friendly, energy ecient, and has a significantly longer life span (>50,000hours
versus 300 hours for a typical mercury bulb and 1,500hours for a metal halide bulb).
LED light cubes
Each user-interchangable, auto-configured light cube contains an LED, collimating optics, and filters.
In addition to the channel dedicated to the transmitted light from the condenser for brightfield contrast
applications, the EVOS
™
M5000 Imaging System can accommodate up to four fluorescent or specialty
light cubes for multi-fluorescence research applications.
The table below lists some of the common fluorescence and specialty light cubes available from
Thermo Fisher Scientific. For a complete list of available light cubes and to inquire about custom light
cubes, go to thermofisher.com/evos or contact Technical Support.
Light cube Dye
DAPI DAPI, Hoechst
™
, BFP
TagBFP TagBFP
CFP ECFP, Lucifer Yellow, Evans Blue
GFP GFP, Alexa Fluor
™
488, SYBR
™
Green, FITC
YFP EYFP, acridine orange + DNA
RFP RFP, Alexa Fluor
™
546, Alexa Fluor
™
555, Alexa Fluor
™
568, Cy
™
3, MitoTracker
™
Orange, RhodamineRed
™
, DsRed
Texas Red
™
Texas Red
™
, Alexa Fluor
™
568, Alexa Fluor
™
594, MitoTracker
™
Red, mCherry,
Cy
™
3.5
AppendixCSystem overview
Operation principles and technical overview
C
136
EVOS
™
M5000 Imaging System User Guide