Glossary
Software controls and functions
•
Autofocus: The Autofocus button triggers the microscope to move through a Z-axis range
(defined by the triangle sliders in the Coarse focus control) and search for the optimal focal plane.
The display shows a live view as the objective sweeps through the defined Z-axis range. Autofocus
uses an image-based focusing algorithm to achieve the desired focus.
Note: Do NOT use autofocus with oil-immersion objectives, as this can create air bubbles in the oil
or cause the objective to collide with the sample.
•
Brightness, Contrast, and Gamma: The Image Display Settings panel provides slider controls
for adjusting the brightness, contrast, and gamma of the current image. These controls can be
used to darken or lighten the image and increase or decrease contrast. The Gamma slider allows
for non-linear contrast adjustments. Moving this slider to the left can help reduce undesired
background fluorescence, particularly in images with nonspecific background fluorescence.
Conversely, moving this slider to the right can enhance fluorescence signals and make them more
visible, especially in images with very faint signals, and can be particularly useful for detecting
signals at the lower limits of brightness.
•
Keyboard shortcuts: Keyboard shortcuts are helpful for eciently performing instrument functions
without relying on mouse actions. These shortcuts typically involve holding down the Control
key and pressing a designated key to trigger a specific action. Keyboard shortcuts for the
EVOS
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M5000 instrument can be used for a range of tasks, including selecting the illumination
channel, toggling the current channel on and o, running autofocus, capturing the current channel,
capturing all selected channels, and saving the displayed image using the current Save settings.
By familiarizing yourself with these shortcuts you can streamline your workflow and enhance
productivity while operating the instrument.
•
Simple and Advanced illumination controls: Simple illumination operates with one slider using
an intelligent algorithm to adjust the LED brightness, exposure time, and camera gain at the
same time. Advanced illumination allows control of the same parameters using 3 separate sliders.
Clicking the icon with 3 sliders from the default Simple illumination control (1 slider) switches to the
Advanced option.
Note: The Simple illumination single slider uses an intelligent algorithm designed to maximize the
signal-to-noise ratio and minimize photobleaching. For most imaging work we recommend using
the single slider to adjust the illumination. For more advanced users it may be useful to control
these settings individually, but please note it is also possible to photobleach the sample or degrade
images if you use the wrong combination of settings.
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M5000 Imaging System User Guide
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