Invitrogen EVOS M5000 - Batch Analysis; Batch Analysis Function; Save Current Analysis Settings; Run Batch Analysis

Batch analysis
Batch Analysis function
Batch Analysis allows you to save and apply the analysis parameters set in Auto Count, Confluence,
and Transfection Eciency tools to other images that you have collected and saved in an image
folder.
The Batch Analysis function is available as a Save Settings-Run Analysis split-button on the Auto
Count and Cell Culture panels on the Review tab. It is not available for Manual Count.
IMPORTANT! Images to be batch analyzed should all be of the same cell type and have the same
magnification
and illumination settings. For consistent measurements, do not mix dierent cell types,
magnifications, or illumination settings in the same folder when performing batch analysis.
IMPORTANT! For batch analysis, only use 16‑bit image
files (TIFF or PNG) that contain the full
dynamic range and metadata needed for quantitative analysis.
Save current analysis settings
1.
When
finished with Auto Count, Confluence measurement, or Transfection Eciency
calculation, click Batch, then select Save Settings.
2.
Enter the name for the settings, then click Save. The current analysis settings are saved for reuse,
which you can apply t
o other images that you have collected and saved an image folder.
Run batch analysis
1.
Navigate to the folder that contains the images you wish to analyze using the Batch Analysis tool,
then click on a representative image to open it.
2.
Perform Auto Count, Confluence measurement, or
Transfection Eciency calculation on the
open image as described previously.
3.
To analyze the remaining images in the same image folder, click Batch, then select Run Analysis.
Note:
You can also directly run Batch Analysis for images in an image folder using previously
saved Batch Analysis settings without first performing Auto Count, Confluence measurement, or
Transfection
Eciency calculation (see Step 4 on page95).
Chapter10Review and analyze saved images
Batch analysis
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EVOS
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M5000 Imaging System User Guide