Invitrogen EVOS M5000 - Page 93

3.
Select Save as screenshot to preserve a detailed account of your count results as an image.
When Save as screenshot is not selected, the software only saves the image as displayed in the
Viewing area. Table1 lists the various options available for saving analysis results.
Table1Save options for analysis tools.
 Save Save as screenshot Save data
Auto Count
Image and object count;
counted objects and
user selected target and
background areas identified
Image (same as Save) and Auto
Count tab with object count,
histogram, and count options as
displayed in the Auto Count tool
Separate image
(same as Save)
and CSV files;
CSV file contains
brightness, area,
and circularity
data
Manual Count
Image and total object count;
object labels as tagged by
user during count
Image (same as Save) and Manual
Count tab with labels, object
counts, percentages, and total
count as displayed in the Manual
Count tool
Not applicable.
Confluence
Image in transmitted light
channel (with or without
mask) and %confluence;
user selected target and
background areas identified
Image (same as Save) and Cell
Culture Confluence tab with %
confluence and sensitivity as
displayed in the Confluence tool
Not applicable.
Transfection
Eciency
Image in selected channels
(with or without mask),
%confluence, and
% transfection eciency in
the fluorescence channel
Image (same as Save) and Cell
Culture Transfection Eciency tab
with %confluence, % transfection
eciency, threshold, and other
options as displayed in the
Transfection Eciency tool
Not applicable.
4.
If you have performed an
Auto Count, select Save data to save a CSV file of your count results
with individual object brightness, area, and circularity data.
This option is not available with Manual Count, Confluence measurement, or Transfection
Eciency calculation.
5.
Click
Save to save your analysis results.
Chapter10Review and analyze saved images
Save analysis results
10
EVOS
™
M5000 Imaging System User Guide
93