Invitrogen EVOS M5000 - Optional: Adjust Display Settings; Find a Region of Interest

Note: Z-Oset specifies the focus position in the selected channel relative to the focus position in
other channels. Setting the correct Z-Oset is especially important when the fluorescent markers in
dierent channels are in dierent focal planes.
Optional: Adjust display settings
1.
If not already visible, hover the pointer over the Viewing area to reveal the buttons for Display
Settings and Analysis Tools.
2.
Click (Image Display Settings)
to expand the controls for image display settings for the
selected channel.
3.
Adjust (Brightness) , (Contrast), and (Gamma) using the corresponding sliders.
4.
Click (Reset) to return the display settings to their default values.
5.
Click (Image Display Settings)
again to collapse the controls.
Note:
The
Gamma slider allows non-linear contrast adjustments; moving it to the left suppresses
low intensities whereas moving it to the right boosts low intensities. For images with nonspecific
background fluorescence (often called "autofluorescence") moving the Gamma slider to the left may
help reduce undesired background fluorescence. For images with very faint fluorescence signals,
moving the Gamma slider to the right may help increase faint signals and bring them out of the
background, making them easier to see or detect at the lower limits of brightness.
Find a region of interest
1.
Use the
X-axis and Y-axis positioning knobs (“Front view” on page13) to move the sample stage
and bring the region of interest into the field of view.
2.
To zoom in and out of the Viewing area, use the Zoom slider. The zoom range is 100–1000%.
You can also double-click on the image to center and zoom by 200% on the clicked location, to a
maximum of 1000%. Right-clicking on a zoomed image r
esets the zoom to 100%.
3.
If needed, readjust the brightness and focus, then proceed to capture the image.
Chapter4Capture images
Capture images in a single channel
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EVOS
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M5000 Imaging System User Guide
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