Invitrogen EVOS M5000 - CHAPTER 5 Measure, Annotate, and Analyze Captured Images; Display Settings and Analysis Tools

Measure, annotate, and analyze
captured images
Display settings and analysis tools
Display settings and analysis tools allow you to change image display settings for live and captured
images in the Viewing area, correct pixel shifts that can occur at higher magnifications in multichannel
fluorescent images, annotate captured images, and perform cell count, measure confluence, and
calculate transfection eciency.
1.
If not already showing, hover the pointer over the Viewing area to reveal the buttons for Display
Settings and Analysis Tools.
Note: Display Settings and Analysis Tools
is available in both the Capture and Review tabs.
2.
Click a button to open the corresponding tool; click the button again to close it. Using the Display
Settings and Analysis Tools, you can:
•
Configure
display settings (“Configure display settings” on page88)
–
Adjust image display settings (“Adjust image display settings” on page37)
–
Display a grid (“Display grid” on page38)
–
Display a scale bar (“Display scale bar” on page39)
•
Align channels (“Align channels” on page40)
Note:
The button to display the Align Channels
tool (indicated by the red arrow) is visible
only if the Show Align Channels in Display Settings and Analysis Tools option is selected in
the Settings4General tab (“Show Align Channels tool” on page102).
•
View pixel intensity histogram (“View pixel intensity histogram” on page41
)
•
Add and show measurements and annotations (“Add measurements and annotations” on
page42)
5
36
EVOS
™
M5000 Imaging System User Guide