Invitrogen EVOS M5000 - Settings

5.
Fluorescence Channel: Toggles the display of the fluorescence channel.
6.
Transmitted Light Channel
: Toggles the display of the transmitted light.
7.
Threshold
: Adjusts the fluorescence threshold value. Only the cells that express above the set
threshold are used in the tranfection eciency calculation.
8.
Threshold Mask
: Indicates the areas above the set threshold value and are included in the
transfection eciency calculation.
9.
% Confluence
and %Transfection Eciency: Displays the calculated confluence and
transfection eciency values.
10.
Reset: Resets the
Confluence and Transfection Eciency measurements to 0 and clears the
selected targets and background areas.
11.
Exit: Exits the Cell Culture tool and displays the Review tab.
12.
Save
: Saves the analysis results as an image in the selected file format (see “Save analysis results”
on page92).
13.
Mask Color: Selects the threshold mask color.
Settings
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AppendixBGraphical user interface (GUI)
Settings
B
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EVOS
™
M5000 Imaging System User Guide