Invitrogen EVOS M5000

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Light cube Dye

Cy5

™

5, Alexa Fluor

™

647, Alexa Fluor

™

660, DRAQ5

™

5.5 Cy

™

5.5, Alexa Fluor

™

660, Alexa Fluor

™

680, Alexa Fluor

™

700

™

7 Cy

™

7, IRDye

™

800CW

Specialty light cubes Dye

CFP-YFP em CFP/YFP (for FRET applications)

AO Acridine orange + RNA, simultaneous green/red with FL color

White Reﬂected light applications

AppendixCSystem overview

Operation principles and technical overview

EVOS

™

M5000 Imaging System User Guide

137

Main Page

default chapter3
- Table of Contents3
CHAPTER 1 about this Guide9
- Audience9
- User Attention Words9
- Safety Alert Words9
CHAPTER 2 Product Information10
- Product Description10
- EVOS ™ M5000 Imaging System10
- EVOS ™ M5000 Software10
- Product Use11
- Standard Items Included11
- EVOS ™ M5000 Imaging System11
- EVOS ™ M5000 Accessories Kit11
- EVOS ™ M5000 Imaging System User Documentation12
- Instrument Exterior Components and Mechanical Controls13
- Front View13
- Rear View14
- Graphical User Interface (GUI)15
- GUI Layout15
CHAPTER 3 Installation16
- Operating Environment and Site Requirements16
- Cell Culture Hood Setup16
- Prepare for Installation17
- Receive and Inspect the Shipment17
- Move the Instrument to the Installation Site17
- Install the Instrument17
- Unpack the Instrument17
- Remove Shipping Restraints18
- Install the UV Light Shield20
- Connect the Instrument22
- Turn on the EVOS ™ M5000 Imaging System22
- Connect the Instrument to the Internet22
- Set Date and Time22
- Connect to the Thermo Fisher ™ Connect Platform23
- About the Thermo Fisher ™ Connect Platform23
- Create a Connect Account23
- Create a PIN Number23
- Link Instrument to Your Connect Account24
- Add Instrument to Your Connect Account with QR Code (Mobile Device)25
- Add Instrument to Thermo Fisher ™ Connect Platform with Linking Code (PC)25
- Sign in to Your Connect Account from the EVOS ™ M5000 Instrument25
- Set up a New Administrator26
CHAPTER 4 Capture Images27
- Overview27
- Workflow27
- Capture Tab28
- Capture Images in a Single Channel28
- Select Objective and Light Source28
- Adjust Brightness29
- Focus on the Sample30
- Optional: Adjust Display Settings31
- Find a Region of Interest31
- Capture Images for each Channel32
- Capture Images in Multiple Channels34
- Capture Multiple Channels Automatically34
CHAPTER 5 Measure, Annotate, and Analyze Captured Images36
- Display Settings and Analysis Tools36
- Adjust Image Display Settings37
- Display Grid38
- Display Scale Bar39
- Align Channels40
- View Pixel Intensity Histogram41
- Display Histogram41
- Add Measurements and Annotations42
- Analyze Cell Culture44
- Analysis Tools44
- Count Cells - Auto Count45
- Perform Auto Count45
- Count Cells - Manual Count51
- Perform Manual Count51
- Measure Confluence54
- Confluence Tool54
- Calculate Transfection Efficiency58
CHAPTER 6 Save Captured Images62
- Save62
- Save Images Manually62
- Quick Save Images63
- (Optional) Enable Quick Save63
CHAPTER 7 Capture Time Lapse Images65
- Time Lapse Tool65
- Run a Time Lapse Routine65
- Run a New Time Lapse Routine65
- Run a Saved Time Lapse Routine70
- Review Images and Video Captured in a Time Lapse Routine71
CHAPTER 8 Capture Z-Stack74
- Z-Stack Tool74
- Capture Z-Stack Images74
CHAPTER 9 Stage View76
- Overview76
- Workflow77
- Set Stage Origin78
- Mark Pin Locations81
- Find Pins Using the Find Target Screen81
- Load Pin Map84
- Update the Target Pin Location84
CHAPTER 10 Review and Analyze Saved Images85
- Review Images86
- Configure Display Settings88
- Adjust Display Settings for Saved Images88
- Display Grid89
- Display Scale Bar89
- View Pixel Intensity Histogram89
- Display Histogram89
- Add Measurements and Annotations to Saved Images90
- Analyze Cell Culture Using Saved Images90
- Save Analysis Results92
- Save92
- Batch Analysis94
- Batch Analysis Function94
- Save Current Analysis Settings94
- Run Batch Analysis94
CHAPTER 11 Configure Instrument Settings96
- Overview96
- Settings Tab96
- Adjust Objective Settings98
- Assign Objectives98
- Calibrate Objective Magnification99
- Delete Objectives99
- Calibrate White Balance100
- Set Saturated Pixel Options101
- Highlight Saturated Pixels101
- General Settings101
- General Settings Options101
- Define Capture All Checkbox Behavior101
- Define Display Settings Toolbar Behavior102
- Show Align Channels Tool102
- Configure Network Settings102
- Connect to a Wi-Fi Network103
- Map Network Drive103
- Service105
- Copy Error Logs105
- Set Date and Time105
- Update from Cloud106
- Update from USB106
CHAPTER 12 Instrument Care and Maintenance107
- General Care107
- Objective Lens Care108
- Stage Care108
- Decontamination Procedures108
- Change EVOS ™ LED Light Cubes109
- Change LED Light Cube109
- Change the Objectives110
- Procedure for Objective Change110
- Calibrate the Objectives112
- Calibrate Objective Magnification112
APPENDIX A Troubleshooting114
- Image Quality Issues114
- Stage View Issues115
- Software Interface Issues115
- Mechanical Issues116
APPENDIX B Graphical User Interface (GUI)117
- Capture Tab117
- Review Tab126
- Auto Count Controls128
- Manual Count Controls129
- Cell Culture - Confluence Controls130
- Cell Culture - Transfection Efficiency Controls131
- Settings132
APPENDIX C System Overview135
- Technical Specifications135
- Physical Characteristics135
- Hardware135
- Operation Principles and Technical Overview136
- LED Illumination136
- LED Light Cubes136
APPENDIX D EVOS ™ Image Analysis138
- EVOS Image Analysis138
- Gallery139
- Edit Image139
- Adjust Tab140
- Analyze Tab141
APPENDIX E Glossary142
- Software Controls and Functions142
- Image Files143
- Microscope Controls and Optics145
- Stage View Feature146
APPENDIX F Safety147
- Symbols on this Instrument147
- Standard Safety Symbols147
- Location of Labels149
- Control and Connection Symbols149
- Conformity Symbols149
- Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific150
- Instrument Safety150
- General150
- Physical Injury151
- Electrical Safety152
- Cleaning and Decontamination153
- Instrument Component and Accessory Disposal153
- Safety and Electromagnetic Compatibility (EMC) Standards153
- Safety Standards154
- EMC Standards154
- Environmental Design Standards155
- Chemical Safety156
- Biological Hazard Safety157
APPENDIX G Documentation and Support158
- Customer and Technical Support158
- Limited Product Warranty158

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Invitrogen EVOS M5000 - Page 137

Table of Contents

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