Invitrogen EVOS M5000 - Page 144

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Pseudocolor image: A pseudocolor image is a modified version of a grayscale image that uses
a single color (for a fluorescence channel) or multiple distinct colors (for a fluorescence overlay
image) to represent the image as it would be seen through a microscope's oculars. The chosen
color(s) typically correspond to the emission wavelength of the fluorescence channel used to
capture the image. With the EVOS
™
M5000 instrument, which uses a highly sensitive monochrome
camera, all fluorescence images are initially captured as monochrome. These images can then be
saved as either monochrome 16-bit images for analysis or pseudocolored 8-bit images for display
purposes. It's important to note that any saved pseudocolor image is always considered to be a
display image.
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TIFF image file: TIFF is an acronym for "tagged image file format", a common digital image file
encoding standard. "Tags" consist of information (also known as metadata) stored in the image
file along with the image. TIFF images for microscopy can contain tags about the objective,
illumination info, camera, exposure time, and other details of image capture. TIFF files are not
compressed and can be saved in analysis or display format. EVOS
™
TIFF files have the following
naming conventions:
–
Basefilename_quicksavenumber_channel_well.ti = 8-bit pseudocolor channel for display
–
Basefilename_quicksavenumber_channel_well.tif = 16-bit monochrome channel for analysis
–
Basefilename_quicksavenumber_well.tif = 8-bit, RGB multi-channel pseudocolor overlay for
display
Note: Bolded text in the file names indicates variables that change between the types.
•
PNG image file: PNG is an acronym for "portable network graphics", a common digital image
file encoding standard. Like TIFF files, PNG files contain information (known as "chunks") stored
along with the image. PNG images for microscopy can contain chunks (that is, metadata) about the
objective, illumination information, camera, exposure time, and other details of image capture. PNG
files use lossless compression and can be saved in analysis or display format. EVOS
™
PNG files
have the following naming conventions:
–
Basefilename_quicksavenumber_channel_well_D.PNG = 8-bit pseudocolor channel for
display
–
Basefilename_quicksavenumber_channel_well.png = 16-bit monochrome channel for analysis
–
Basefilename_quicksavenumber_well.png = 8-bit RGB multi-channel pseudocolor overlay for
display
Note: Bolded text in the file names indicates variables that change between the types.
•
JPEG image file: JPEG is an acronym for "joint photographics experts group", a common digital
image file encoding standard. As opposed to TIFF or PNG files, JPEG image files are often highly
compressed, which results in data loss. In addition to lossy compression, standard JPEG images
do not contain metadata and have limited dynamic range (only 8-bit images). These images are
useful for display, sharing, and viewing and can easily be shared and viewed, however, they are not
optimal for analysis. EVOS
™
JPEG files have the following naming conventions:
–
Basefilename_quicksavenumber_channel_well.jpg = 8-bit pseudocolor channel for display
–
Basefilename_quicksavenumber_well.jpg = 8-bit RGB multi-channel pseudocolor overlay for
display
Note: Bolded text in the file names indicates variables that change between the types.
AppendixEGlossary
Image files
E
144
EVOS
™
M5000 Imaging System User Guide