Invitrogen EVOS M5000 - Page 5

Count cells – manualcount ...................................................... 51
Perform manualcount ...................................................... 51
Measureconfluence ............................................................ 54
Confluence tool ........................................................... 54
Calculate transfectioneciency ............................................. 58
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CHAPTER6Save capturedimages .............................................. 62
Save ......................................................................... 62
Save imagesmanually ...................................................... 62
Quick Saveimages ............................................................. 63
(Optional) Enable QuickSave ................................................ 63
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CHAPTER7Capture time lapseimages ........................................ 65
Time lapse tool ................................................................ 65
Run a time lapse routine ........................................................ 65
Run a new time lapse routine ................................................ 65
Run a saved time lapse routine .............................................. 70
Review images and video captured in a time lapse routine ...................... 71
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CHAPTER8Capture Z-Stack .................................................... 74
Z-Stack tool ................................................................... 74
Capture Z-stackimages ........................................................ 74
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CHAPTER9Stage View .......................................................... 76
Overview ...................................................................... 76
Workflow ................................................................. 77
Set StageOrigin ............................................................... 78
Mark pinlocations ............................................................. 81
Find pins using the Find Target screen ............................................ 81
Load PinMap ................................................................. 84
Update the target pinlocation ................................................... 84
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CHAPTER10Review and analyze savedimages .............................. 85
Reviewimages ................................................................ 86
Reviewimages ............................................................ 86
Configure displaysettings ....................................................... 88
Adjust display settings for savedimages ...................................... 88
Displaygrid ............................................................... 89
Display scalebar .......................................................... 89
View pixel intensity histogram ................................................... 89
Display histogram .......................................................... 89
Contents
EVOS
™
M5000 Imaging System User Guide
5