Invitrogen EVOS M5000 - Page 7

Change theobjectives ......................................................... 110
Procedure for objectivechange ............................................ 110
Calibrate theobjectives ........................................................ 112
Calibrate objectivemagnification ........................................... 112
■
APPENDIXATroubleshooting .................................................. 114
Image qualityissues ........................................................... 114
Stage viewissues ............................................................. 115
Software interfaceissues ...................................................... 115
Mechanicalissues ............................................................ 116
■
APPENDIXBGraphical user interface(GUI) ................................... 117
Capturetab .................................................................. 117
Reviewtab ................................................................... 126
Auto count controls ........................................................... 128
Manual count controls ......................................................... 129
Cell culture – confluence controls ............................................... 130
Cell culture – transfection eciency controls ..................................... 131
Settings ..................................................................... 132
■
APPENDIXCSystem overview ................................................. 135
Technicalspecifications ........................................................ 135
Physical characteristics ................................................... 135
Hardware ................................................................ 135
Operation principles and technical overview ...................................... 136
LEDillumination .......................................................... 136
LED lightcubes .......................................................... 136
■
APPENDIXDEVOS
™
imageanalysis ........................................... 138
EVOS
™
imageanalysis ......................................................... 138
Gallery ...................................................................... 139
Editimage ................................................................... 139
Adjusttab ............................................................... 140
Analyzetab .............................................................. 141
■
APPENDIXEGlossary ........................................................... 142
Software controls andfunctions ................................................ 142
Imagefiles ................................................................... 143
Microscope controls andoptics ................................................. 145
Stage View feature ............................................................ 146
Contents
EVOS
™
M5000 Imaging System User Guide
7