Invitrogen EVOS M5000 - Page 91

2.
Click to select from the following analysis options:
•
Auto Count: Automatically counts the objects displayed in the Viewing area based on your
specifications. With Auto Count, you can only count objects in a single fluorescence channel
(typically a nuclear stain channel).
For instructions on how to perform Auto Count, see “Count cells – auto count” on page45.
•
Manual Count: Allows you to tag objects in the Viewing area with up to six labels. As you
tag objects, the system keeps a running tally of the counts with percentages for each label
assigned. With Manual Count, you can count objects in multiple channels simultaneously.
For instructions on how to perform Manual Count, see “Count cells – manual count” on
page51.
•
Cell Culture: Allows you to measure the confluence of your culture and calculate the
transfection eciency.
–
Confluence: Allows you to select up to 5 reference objects for the target (i.e., cells) and
one background reference in your image, then automatically calculates the percentage
confluence of your culture.
For instructions on how to measure the confluence of your culture, see “Count cells –
manual count” on page51.
–
Transfection Eciency: Allows you to estimate the transfection eciency of your culture
by calculating the ratio of fluorescence area (i.e., cells expressing the fluorescence
marker) to the total cell area in your culture.
For instructions on how to calculate the transfection eciency, see “Calculate transfection
eciency” on page58.
3.
If desired, perform
Batch Analysis to the remainder of the images in the image folder (“Save
analysis results” on page92).
Note:
Batch Analysis allows you to save and apply the analysis parameters set in Auto Count,
Confluence, and Transfection
Eciency tools to other images that you have saved in an image
folder. Batch Analysis is not available for Manual Count.
Chapter10Review and analyze saved images
Analyze cell culture using saved images
10
EVOS
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M5000 Imaging System User Guide
91