Maintenance and Troubleshooting 107
Troubleshooting the Protein Application
7
Symptoms (Protein)
Low Ladder Peaks
Back to “Symptoms (Protein)” on page 89
Most probable causes Solution
Ladder degraded. Refer to the Kit Guide for proper ladder storage. Optional: Prepare ladder aliquots
and use a new aliquot.
Ladder not diluted after denaturing. Refer to Kit Guide for proper chip preparation.
Probable causes Solution
Improper denaturation of ladder. Use fresh ladder aliquot. Heat ladder for 5 minutes at 100°C.
Use 0.5 mL tubes for denaturation.
Diluted ladder is too old. Use diluted ladder within one day.
Pipetting error during preparation of
reagent mixes.
Check dilution procedure and calibration of pipette.
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