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Agilent Technologies 2100 Maintenance And Troubleshooting Guide
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7
Maintenanc
e and
Troubleshooting
Agilent T
echnologies
1
Essential Measurement Practices
Overview
8
T
ools and Handling
9
Chip Priming Station
10
Reagents and Reagent Mix
es
11
Gel and Gel-Dye Mix
11
Samples
12
Chips
13
Agilent 2100 Bioanalyzer System
14
6
8
Table of Contents
Table of Contents
4
Safety Notices
2
1 Essential Measurement Practices
7
Overview
8
Tools and Handling
9
Chip Priming Station
10
Reagents and Reagent Mixes
11
Samples
12
Chips
13
Agilent 2100 Bioanalyzer System
14
2 Troubleshooting the Instrument Communication
15
Default Chapter
16
Verify the Instrument Communication
16
Troubleshooting Communication Issues
18
Overview
18
Troubleshooting Communication Issue Flow Chart
19
Changing COM Port Settings
20
USB to Serial Adapter
21
How to Create a Support Package
22
3 Troubleshooting the 2100 Expert Software Run Installation Qualification Test
26
4 Hardware Diagnostics Overview
31
Diagnostic Test Procedure
32
5 Troubleshooting the Dna Application
35
Overview
36
Symptoms (DNA)
37
Symptoms (DNA)
38
Residual Gel in Spin Filter after Centrifugation
38
Too High Quantitation Results
38
Too Low Quantitation Results
39
Wrong Sizing Result
40
Run Aborted
41
Chip Not Detected
42
Additional Sample or Ladder Peaks
43
Spikes
44
Low Signal Intensity
45
Missing Peaks
47
Missing Upper Marker
48
Broad Peaks
49
Baseline Dips
50
Baseline Noise
51
Baseline Jumps
52
Wavy Baseline
53
Late Migration
54
Peak Tailing
56
Unexpected Run Time
57
Error Message: no Data Received Since 5 Seconds
61
Symptoms (High Sensitivity DNA)
62
Artefact Peaks
63
Split Peaks
64
Baseline Negative Dips
65
6 Troubleshooting the Rna Application
67
Overview
68
Symptoms (RNA)
69
Residual Gel in Spin Filter after Centrifugation
70
Too High Quantitation Results
70
Too Low Quantitation Results
71
Chip Not Detected
72
Additional Sample or Ladder Peaks
74
Spikes
77
Low Signal Intensity
78
Missing Peaks
81
Troubleshooting the DNA Application
85
Symptoms (DNA)
85
Symptoms (RNA)
86
7 Troubleshooting the Protein Application
87
Overview
88
Symptoms (Protein)
89
Residual Gel in Spin Filter after Centrifugation
90
Too High Quantitation Results
90
Too Low Quantitation Results
91
Chip Not Detected
93
Run Aborted
94
Additional Sample or Ladder Peaks
99
Spikes
104
Low Signal Intensity
106
Broad Peaks
108
Late Migration
110
Symptoms (High Sensitivity Protein)
112
8 Maintenance of the Electrode Cartridge
115
Overview
116
DNA and Protein Assays
117
Cleaning the Electrodes after each DNA and Protein Assays
118
RNA Nano Assay
119
Cleaning the Electrodes before each RNA Nano Assay
120
Cleaning the Electrodes after each RNA Nano Assay
121
Rnase Decontamination of the Pin Set
122
RNA Pico or Small RNA Assay
123
Cleaning the Electrodes before each RNA Pico or Small RNA Assay
124
Cleaning the Electrodes after each RNA Pico or Small RNA Assay
125
Rnase Decontamination of the Pin Set
125
How to Clean the Pin Set of the Electrode Cartridge
126
9 Maintenance of the Chip Priming Station
131
Default Chapter
132
Overview
132
Replacing the Syringe
133
Cleaning the Syringe Adapter
134
Replacing the Syringe Adapter
136
Replacing the Gasket
137
Checking the Chip Priming Station for Proper Performance - Seal Test
139
10 Maintenance of the Agilent 2100 Bioanalyzer Instrument
141
Overview
142
Cleaning the Lens
143
Changing the Fuses
144
11 Maintenance of the Vortexer Changing the Adapter
150
Changing the Adapter
150
12 Spare Parts and Accessories
151
Overview
152
Other manuals for Agilent Technologies 2100
User Guide
366 pages
Installation And Safety Guide
42 pages
5
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Agilent Technologies 2100 Specifications
General
Brand
Agilent Technologies
Model
2100
Category
Measuring Instruments
Language
English
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