Maintenance and Troubleshooting 71
Troubleshooting the RNA Application
6
Symptoms (RNA)
Too Low Quantitation Results
Back to “Symptoms (RNA)” on page 69.
Most probable causes Solution
Reference measurement (e.g. UV
absorption) was elevated due to
contaminants in sample.
Purify sample prior to measurement.
Pipetting error during preparation of
ladder, samples, or reagent mixes.
Use appropriate calibrated pipette and tips.
Check dilution procedure.
Always insert the pipette tip to the bottom of the well when dispensing the
liquid.
Insufficient vortexing of chip. Vortex chip for 1 minute. Only use the IKA vortexer. Adjust the speed to the
set-point.
Probable causes Solution
RNA ladder not denatured. Heat denature the RNA ladder as described in the Kit Guide.
Sample concentration too high. Use the sample concentration recommended by the Kit Guide.
Least probable causes Solution
Dye concentration too high. Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes before preparing the gel-dye mix. Protect the dye
from light during this time.
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