Maintenance and Troubleshooting 13
Essential Measurement Practices
1
Chips
Chips
• Prepared chips must be used within 5 minutes. Reagents may evaporate,
leading to poor results.
• For DNA and RNA assays, vortex chips for 1 minute. Inappropriate and
insufficient vortexing will lead to poor results. Use only the IKA vortexer
for chip vortexing. Replace the chip adapter if it is worn out. For the MS-2
vortexers with 3 mounting screws, the replacement part number is
5065-9966. For MS-3 vortexers with 4 mounting screws, replacement
adapters may be purchased directly from IKA (www.ika.de) with part
number 3428300.
• Do not touch the wells of the chip. The chip could get contaminated
resulting in poor measurement results.
• Do not leave any wells of the chip empty. The assay will not run properly.
For DNA and RNA assays: Add 1 μL of sample buffer to each unused sample
well so the total liquid volume in each well is at least 6 μL.
For protein assays: pipette a sample or ladder replicate in any empty
sample well.
• Do not touch the underside of the chip.
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