38 Maintenance and Troubleshooting
5
Troubleshooting the DNA Application
Symptoms (DNA)
Residual Gel in Spin Filter after Centrifugation
Too High Quantitation Results
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Gel was filtered at insufficient g-value. Refer to the Kit Guide for proper centrifuge settings.
Cooled centrifuge was used for gel
filtration.
Repeat centrifugation step at room temperature.
Gel was too cool or viscous. Reagents must be equilibrated at room temperature for 30 minutes prior to use.
Most probable causes Solution
Pipetting error during preparation of
reagent mixes.
Check dilution procedure and check calibration of pipette.
Chip pipetting error. Prepare new chip. Always insert the pipette tip to the bottom of the well when
dispensing the liquid. Use appropriate pipette and tips.
Insufficient vortexing of chip. Vortex chip for 1 minute. Only use the IKA vortexer. Adjust the speed to the
set-point.
Probable causes Solution
Dye concentration too low (marker
disappears).
Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes before preparing the gel-dye mix. Protect dye from
light during this time.
Low or missing upper marker. Check “Missing Upper Marker” on page 48.
Least probable causes Solution
Loaded chip kept for too long before
run.
Prepared chips must be used within 5 minutes.
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