90 Maintenance and Troubleshooting
7
Troubleshooting the Protein Application
Symptoms (Protein)
Residual Gel in Spin Filter after Centrifugation
Too High Quantitation Results
Back to “Symptoms (Protein)” on page 89
Most probable causes Solution
Gel was filtered at insufficient g-value. Refer to the Kit Guide for proper centrifuge settings.
Cooled centrifuge was used for gel
filtration.
Repeat centrifugation step at room temperature.
Gel was too cool or viscous. Reagents must be equilibrated at room temperature for 30 minutes prior to use.
Most probable causes Solution
Alignment of upper marker not set
properly.
Manually set upper marker. Follow instructions for “Manual Marker
Assignment” on page 96 .
Pipetting error during preparation of
reagent mixes or chip.
Refer to the Kit Guide for proper preparation of reagents. Check dilution
procedure and calibration of pipette.
Chip preparation error. Prepare new chip. Always insert the pipette tip to the bottom of the well when
dispensing the liquid. Use appropriate pipette and tips.
Probable causes Solution
Upper marker is degraded by
proteases.
Treat sample with protease inhibitors prior to sample preparation.
Sample salt concentration is too high. Check maximum sample buffer salt limits in the compatible buffer list in the Kit
Guide. Dilute the sample prior to the sample preparation or use a different buffer,
if possible.
Improper denaturation of sample. Use fresh sample aliquot. Heat sample or denaturing solution for 5 minutes at
100°C.
Use 0.5 mL tubes for denaturation.
Least probable causes Solution
Loaded chip kept too long before run. Prepared chips must be used within 5 minutes of preparation.
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