Maintenance and Troubleshooting 47
Troubleshooting the DNA Application
5
Symptoms (DNA)
Missing Peaks
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Sample salt concentration is too high. Refer to the maximum sample buffer salt limits as specified in the Kit Guide.
Dilute samples with deionized DNase free water, if necessary.
Chip not properly primed. Clogged chip
priming station or wrong priming
station settings.
Prepare a new chip. Check chip priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Leak currents due to contaminated
electrodes.
Clean electrodes with analysis-grade water and a toothbrush, see “How to Clean
the Pin Set of the Electrode Cartridge” on page 126.
Least probable causes Solution
Laser broken. Perform Optics, Autofocus, and Laser Stability tests as described in “Hardware
Diagnostics” on page 29. If tests fail, contact Agilent Technologies at
www.agilent.com/genomics/contact.
Autofocus failure or high voltage
power supply defective
Check autofocus and high voltage power supply by means of the “Hardware
Diagnostics” on page 29. If a diagnostic test fails, contact Agilent Technologies
at www.agilent.com/genomics/contact.
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