Maintenance and Troubleshooting 41
Troubleshooting the DNA Application
5
Symptoms (DNA)
Run Aborted
In the logbook, an error will appear: Run aborted on port x. Instrument error occurred on
port x, Unusual high or low voltage or current was detected during the start phase of the
on-chip analysis. The marked wells will indicate the wells on the chip that caused the
problem.
Most probable causes Solution
Insufficient volume in well(s). Check Kit Guide for the amount of liquid to be pipetted. Ensure all wells contain sufficient
ladder, samples or buffer.
Air bubble at the bottom of
the well, obstructing access
to microchannels.
Always insert the pipette tip to the bottom of the well when dispensing the liquid. Remove
large air bubbles with a pipette tip (small bubbles on top of the well will not affect the
assay).
Dirty electrodes. Clean electrodes according to instructions in “How to Clean the Pin Set of the Electrode
Cartridge” on page 126.
Chip not properly primed.
Clogged chip priming station
or wrong priming station
settings.
Prepare a new chip. Check chip priming station as described in “Checking the Chip
Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket, and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit Guide).
Probable causes Solution
Chip prepared with cold
reagents.
Prepare a new chip. Allow all reagents and reagent mixes to warm up to room
temperature for 30 minutes prior to use.
Store chips at room temperature.
Least probable causes Solution
High voltage power supply
defective.
Check high voltage power supply using the “Overview” on page 30. If the power supply is
defective, contact Agilent Technologies at www.agilent.com/genomics/contact.
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