78 Maintenance and Troubleshooting
6
Troubleshooting the RNA Application
Symptoms (RNA)
Low Signal Intensity
Back to “Symptoms (RNA)” on page 69
Most probable causes Solution
Gel-dye mix expired. Use prepared gel-dye mix within one day.
Dye concentration too low. Use dye concentration according to the Kit Guide.
Let the dye warm up to room temperature for 30 minutes before preparing the
gel-dye mix. Protect the dye from light during this time.
Pipetting error during preparation of
reagent mixes.
Check dilution procedure and calibration of pipette.
Chip pipetting error. Prepare new chip. Always insert the pipette tip to the bottom of the well when
dispensing the liquid. Use appropriate pipette and tips.
Probable causes Solution
Fingerprint on focusing lens or on the
backside of the chip.
Clean lens as described in “Cleaning the Lens” on page 143.
Do not touch the underside of the chip
Insufficient vortexing of chip. Vortex chip for 1 minute. Only use IKA vortexer. Adjust speed to set-point .
Least probable causes Solution
Autofocus or laser failure. Check autofocus and laser using the “Overview” on page 30. If laser stability
diagnostic tests fail, contact Agilent Technologies at
www.agilent.com/genomics/contact.
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