Maintenance and Troubleshooting 49
Troubleshooting the DNA Application
5
Symptoms (DNA)
Broad Peaks
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Leak currents due to contaminated
electrodes.
Clean electrodes with analysis-grade water and a toothbrush, see “How to Clean
the Pin Set of the Electrode Cartridge” on page 126.
Chip not properly primed. Clogged chip
priming station or wrong priming
station settings.
Prepare a new chip. Check the priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Dye concentration too high. Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes before preparing the gel-dye mix.
Probable causes Solution
Genomic DNA or cDNA contamination. Check DNA preparation procedure.
To Next Page
Loading...
Need help?
General
