120 Maintenance and Troubleshooting
8
Maintenance of the Electrode Cartridge
RNA Nano Assay
Cleaning the Electrodes before each RNA Nano Assay
To avoid decomposition of the RNA sample, follow this electrode
decontamination procedure on a daily basis before running any RNA Nano assay.
1 Slowly fill one of the wells of an electrode cleaner with 350 μL RNaseZAP.
2 Open the lid and place electrode cleaner in the 2100 Bioanalyzer
instrument.
3 Close the lid and leave it closed for about 1 minute.
4 Open the lid and remove the electrode cleaner - label the electrode cleaner
and keep for future use. You can reuse the electrode cleaner for all chips in
one kit.
5 Slowly fill one of the wells of another electrode cleaner with 350 μL
RNase-free water.
6 Place electrode cleaner chip in the 2100 Bioanalyzer instrument.
7 Close the lid and leave it closed for about 10 seconds.
8 Open the lid and remove the electrode cleaner. Label it and keep it for
further use.
9 Wait another 10 seconds for the water on the electrodes to evaporate before
closing the lid.
When Before each RNA Nano run.
Parts required # p/n Description
2 NA Electrode cleaner (required amount included in the kits)
1 NA RNase-free water
1 NA RNaseZAP (Ambion, Inc cat. no. 9780)
Perform the following RNase decontamination procedure on a daily basis before running
any RNA Nano assays.
Leak currents between electrodes
Liquid spill may cause leak currents between the electrodes.
➔ Never fill more than 350 µL of water in the electrode cleaner.
Remove the RNaseZAP and the RNase-free water from the electrode cleaner at the end of
the day.
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