Maintenance and Troubleshooting 77
Troubleshooting the RNA Application
6
Symptoms (RNA)
Spikes
Back to “Symptoms (RNA)” on page 69
Most probable causes Solution
Vibration of 2100 Bioanalyzer
instrument.
Do not touch the 2100 Bioanalyzer instrument during a run.
Remove vibration devices, such as vortexers and vacuum pumps, from the
bench.
Particles in tubes. For reagent preparation, use tubes that are supplied with the kit. Do not use
autoclaved tubes.
Chip or gel-dye mix contaminated with
particles.
Prepare new chip with new gel-dye mix.
Wear powder-free gloves only.
Do not touch the underside of the chip.
Do not touch the wells of the chip.
Clean the electrodes.
Load the chip immediately after taking it out of its sealed bag.
Dye agglomerates present in the
gel-dye mix.
Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes and vortex for 10 seconds before preparing the
gel-dye mix. Protect the dye from light during this time. After centrifugation, the
freshly prepared gel-dye mix should be taken up from the top of the tube.
Probable causes Solution
Chip prepared with cold reagents. Prepare a new chip. Allow all reagents and reagent mixes to warm up to room
temperature for 30 minutes before use.
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