106 Maintenance and Troubleshooting
7
Troubleshooting the Protein Application
Symptoms (Protein)
Low Signal Intensity
Back to “Symptoms (Protein)” on page 89
Most Probable Causes Solution
Protein concentration is too low. Follow specifications given in the Kit Guide.
Sample salt concentration is too
high.
Salt concentration strongly affects the sensitivity of the assay. If necessary dilute,
dialyze or desalt the sample. It is recommended to perform a serial dilution with water
or compatible buffer to determine the optimal dilution.
Insufficient dissolution of SDS in
the dye.
Allow the dye to equilibrate to room temperature for 30 minutes before use. Protect
dye from light during this time. Check for undissolved SDS crystals in the tube. Vortex
dye well before use. If necessary, heat the sample buffer to 37°C for 2 minutes.
Samples were not diluted prior to
chip loading
Dilute samples according to protocol given in the Kit Guide.
Probable Causes Solution
Improper denaturation of
samples.
Prepare fresh sample aliquot. Heat sample and denaturating solution for 5 minutes at
100°C.
Use 0.5 mL tubes for denaturation.
Pipetting error during preparation
of reagent mixes.
Check dilution procedure and check calibration of pipette(s).
Least Probable Causes Solution
Samples dissolved in acidic
buffer.
Neutralize samples with appropriate buffer or dilute samples in deionized H
2
O.
Alternatively, dialyze samples against buffer with medium pH.
To Next Page
Loading...
Need help?
General
