Maintenance and Troubleshooting 125
Maintenance of the Electrode Cartridge
8
RNA Pico or Small RNA Assay
Cleaning the Electrodes after each RNA Pico or Small RNA Assay
When the assay is complete, immediately remove the used chip out of the 2100
Bioanalyzer instrument and dispose of it according to good laboratory
practice. Then perform the following procedure to ensure that the electrodes
are clean and no residues are leftover from the previous assay.
1 Slowly fill one of the wells of an electrode cleaner with 350 μL RNase-free
water.
2 Open the lid and place electrode cleaner in the 2100 Bioanalyzer
instrument.
3 Close the lid and leave it closed for 30 seconds.
4 Open the lid and remove the electrode cleaner.
5 Wait another 30 seconds for the water on the electrodes to evaporate before
closing the lid.
RNase Decontamination of the Pin Set
When the pin set of the electrode cartridge is suspected to be contaminated
with RNases follow the instructions described in “How to Clean the Pin Set of
the Electrode Cartridge” on page 126.
When After each RNA Pico or Small RNA run.
Parts required # p/n Description
1 NA Electrode cleaner (required amount included in the kits)
1 NA RNase-free water
Leak currents between electrodes
Liquid spill may cause leak currents between the electrodes.
➔ Never fill more than 350 µL of water in the electrode cleaner.
Replace the water in the electrode cleaner after each use. Use a new electrode cleaner
after 12-13 electrode cleaning procedures and with each new kit.
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