Maintenance and Troubleshooting 43
Troubleshooting the DNA Application
5
Symptoms (DNA)
Additional Sample or Ladder Peaks
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Chip or gel-dye mix
contaminated with particles.
Prepare new chip with new gel-dye mix:
Wear powder-free gloves only.
Do not touch the underside of the chip.
Do not touch the wells of the chip.
Clean the electrodes.
Load the chip immediately after taking it out of its sealed bag.
Sample degraded or
contaminated.
Always wear gloves when handling chips and samples.
Probable causes Solution
Chip prepared with cold
reagents.
Prepare a new chip. Allow all reagents and reagent mixes to warm up to room temperature
for 30 minutes before use.
Chip not properly primed.
Clogged chip priming station
or wrong priming station
settings.
Prepare a new chip. Check chip priming station, see “Checking the Chip Priming Station for
Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket, and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit Guide).
Vibration of 2100
Bioanalyzer instrument.
Do not touch 2100 Bioanalyzer instrument during a run. Remove vibration devices, such as
vortexers and vacuum pumps, from bench.
Dye agglomerates present in
the gel-dye mix.
Use dye concentration according to the Kit Guide.
Let the dye warm up to room temperature for 30 minutes before preparing the gel-dye mix.
Protect the dye from light during this time.
After centrifugation, the gel-dye mix should be taken up from the top of the tube.
Least probable causes Solution
DNA ladder degraded. Check expiration date of reagents. Use fresh DNA ladder.
To Next Page
Loading...
Need help?
General
