118 Maintenance and Troubleshooting
8
Maintenance of the Electrode Cartridge
DNA and Protein Assays
Cleaning the Electrodes after each DNA and Protein Assays
When the assay run is complete, immediately remove the used chip out of the
2100 Bioanalyzer instrument and dispose of it according to good laboratory
practice. Then perform the following procedure to ensure that the electrodes
are clean and no residues are left over from the previous assay.
1 Slowly fill one of the wells of the electrode cleaner with 350 μL deionized
analysis-grade water.
2 Open the lid and place electrode cleaner in the 2100 Bioanalyzer
instrument.
3 Close the lid and leave it closed for about 10 seconds.
4 Open the lid and remove the electrode cleaner.
5 Wait another 10 seconds for the water on the electrodes to evaporate.
6 Empty the electrode cleaner after every cleaning procedure and refill the
electrode cleaner.
7 After 25 Chip runs, replace the used electrode cleaner with a new one.
When After each DNA and Protein run.
Parts required # p/n Description
1 NA Electrode cleaner (required amount included in the kits)
1 NA Deionized analysis-grade water
Leak currents between electrodes
Liquid spill may cause leak currents between the electrodes.
➔ Never fill more than 350 µL of water in the electrode cleaner.
When switching between different assays, a more thorough cleaning may be required. For
details, refer to “How to Clean the Pin Set of the Electrode Cartridge” on page 126.