48 Maintenance and Troubleshooting
5
Troubleshooting the DNA Application
Symptoms (DNA)
Missing Upper Marker
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Alignment of upper marker not set
properly.
Manually assign upper marker. Follow instructions for “Manual Marker
Assignment” on page 58
Upper marker digested by restriction
enzymes.
Inactivate restriction enzymes by adding EDTA or heat according to the
manufacturer's instructions.
Probable causes Solution
Sample salt concentration is too high. Refer to the maximum sample buffer salt limits as specified in the Kit Guide.
Dilute samples with deionized DNase free water if necessary.
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