40 Maintenance and Troubleshooting
5
Troubleshooting the DNA Application
Symptoms (DNA)
Wrong Sizing Result
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
DNA ladder degraded. Check expiration date of reagents.
Chip not properly primed. Clogged chip
priming station or wrong priming
station settings.
Prepare a new chip. Check chip priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket, and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Chip contaminated. Wear powder-free gloves only.
Do not touch the underside of the chip.
Do not touch the wells of the chip.
Clean the electrodes.
Load the chip immediately after taking it out of its sealed bag.
Markers called incorrectly. Manually assign lower marker. Follow instructions for “Manual Marker
Assignment” on page 58
Probable causes Solution
Loaded chip kept for too long before
run.
Prepared chips must be used within 5 minutes.
No ladder in ladder well. Prepare a new chip.
Least probable causes Solution
Vibration of 2100 Bioanalyzer
instrument.
Do not touch 2100 Bioanalyzer instrument during a run.
Remove vibration devices, such as vortexers and vacuum pumps, from bench.
Changes of ambient temperature of
more than 5°C during the run.
Place 2100 Bioanalyzer instrument in thermally stable environment.
High voltage power supply defective. Check high voltage power supply using the “Overview” on page 30. If the power
supply is defective, contact Agilent Technologies at
www.agilent.com/genomics/contact.
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