50 Maintenance and Troubleshooting
5
Troubleshooting the DNA Application
Symptoms (DNA)
Baseline Dips
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Sample concentration is too high. Use sample concentration according to the Kit Guide.
Sample impurities: e.g. genomic DNA,
ss DNA, etc.
Check DNA-isolation protocol. If possible, clean up samples.
Probable causes Solution
Sample salt concentration is too high. Refer to the maximum sample buffer salt limits as specified in the Kit Guide.
Dilute samples with deionized DNase free water, if necessary.
Dye concentration is too low. Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes before preparing the gel-dye mix. Protect the dye
from light during this time.
Least probable causes Solution
Autofocus failure. Check autofocus by means of the “Overview” on page 30. If the diagnostic test
fails, contact Agilent Technologies at www.agilent.com/genomics/contact.
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