Maintenance and Troubleshooting 93
Troubleshooting the Protein Application
7
Symptoms (Protein)
Chip Not Detected
Back to “Symptoms (Protein)” on page 89
Most probable causes Solution
Insufficient volume in well(s) or chip is
empty.
Check Kit Guide on amount of liquid to be pipetted. Ensure all wells contain
sample, ladder or buffer.
Air bubbles at the bottom of the well. Always insert the pipette tip to the bottom of the well when dispensing the
liquid. Remove large air bubbles with a pipette tip (small bubbles on top of well
do not affect the assay).
Probable causes Solution
No communication between the
instrument and PC.
Test the PC-instrument communication as described in “Verify the Instrument
Communication” on page 16.
Chip not properly primed. Air bubble in
chip.
Prepare a new chip. Check chip priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket, and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Least probable causes Solution
High voltage power supply defective. Check high voltage power supply using the “Overview” on page 30. If the power
supply is defective, contact Agilent Technologies at
www.agilent.com/genomics/contact.
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