87
Maintenance and Troubleshooting
Agilent Technologies
7
Troubleshooting the Protein Application
Overview 88
Symptoms (Protein) 89
Residual Gel in Spin Filter after Centrifugation 90
Too High Quantitation Results 90
Too Low Quantitation Results 91
Wrong Sizing Result 92
Chip Not Detected 93
Run Aborted 94
Unexpected Run Time 95
Additional Sample or Ladder Peaks 99
Missing Upper Marker 101
Broad Variability of the Lower Marker 102
Missing Peaks 103
Spikes 104
Poor Reproducibility 105
Low Signal Intensity 106
Low Ladder Peaks 107
Broad Peaks 108
Baseline Dips 109
Late Migration 110
Error Message: No data received since 5 seconds 111
Symptoms (High Sensitivity Protein) 112
Saturation of Lower Marker or Sample Peaks – Optical Signal too
High 113
Low Signal Intensity 114